product M1255, Calcein Blue to is used to stain cells, but it will stain living or dead cells. For a cell viability stain, one can use Calcein Blue AM. The difference between Calcein Blue and Calcein Blue AM is the methyl-iminodiaceitc acid group that chelates the divalent metal has acetoxymethyl esters on it in Calcein Blue AM. The esters are cleaved inside of viable cells, but remain on the molecule in the absence of active esterases. So, if you use M1255 it will stain all the cell whether alive or dead.
M1255
Calcein Blue
Powder
Sinonimo/i:
4-Methylumbelliferone-8-methyliminodiacetic acid
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
Scegli un formato
Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 1 g | Per conoscere la disponibilità, visualizza il carrello | CHF 107.00 | |
| 10 g | Per conoscere la disponibilità, visualizza il carrello | CHF 513.00 |
Informazioni su questo articolo
Formula empirica (notazione di Hill):
C15H15NO7
Numero CAS:
Peso molecolare:
321.28
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
259-129-8
MDL number:
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartiNome del prodotto
Calcein Blue, fluorescent dye
Quality Segment
form
powder
technique(s)
titration: suitable
color
white to light yellow
solubility
1 M NaOH: 50 mg/mL
fluorescence
λex 322 nm; λem 445 nm in methanol
suitability
suitable for indicator for EDTA titration
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
InChI
1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
InChI key
RJPSHDMGSVVHFA-UHFFFAOYSA-N
General description
Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
Application
Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.
1 of 1
Questo articolo | |||
|---|---|---|---|
| suitability suitable for indicator for EDTA titration | suitability suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg) | suitability - | suitability suitable for microscopy |
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) titration: suitable | technique(s) microbe id | staining: suitable |
| color white to light yellow | color yellow to brown | color - | color - |
| fluorescence λex 322 nm; λem 445 nm in methanol | fluorescence λex 470 nm; λem 509 nm (pH 7.4) | fluorescence - | fluorescence - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility 1 M NaOH: 50 mg/mL | solubility NaOH: 50 mg/mL | solubility - | solubility water: 0.01 g/10 mL, blue to very deep blue |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Articoli
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
Contenuto correlato
Liangbo Hu et al.
Virologica Sinica, 34(4), 454-466 (2019-06-16)
Baculovirus can transduce a wide range of mammalian cells and is considered a promising gene therapy vector. However, the low transduction efficiency of baculovirus into many mammalian cells limits its practical application. Co-expressing heterologous viral glycoproteins (GPs), such as vesicular
Xiaomian Wu et al.
International journal of nanomedicine, 7, 1215-1225 (2012-03-16)
Compared with titanium (Ti) and other metal implant materials, poly(ether-ether ketone) (PEEK) shows outstanding biomechanical properties. A number of studies have also reported attractive bioactivity for nano-TiO(2) (n-TiO(2)). In this study, n-TiO(2)/PEEK nanocomposites were prepared, taking advantage of the unique
Tobias A Fuchs et al.
The Journal of cell biology, 176(2), 231-241 (2007-01-11)
Neutrophil extracellular traps (NETs) are extracellular structures composed of chromatin and granule proteins that bind and kill microorganisms. We show that upon stimulation, the nuclei of neutrophils lose their shape, and the eu- and heterochromatin homogenize. Later, the nuclear envelope
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| M1255-10G | 04061833444788 |
| M1255-1G | 04061826762202 |
-
Is Calcein Blue identical to Calcein Blue AM, and if not, what is the difference? Would it be compatible in a flourescent microscopy assay looking at %viability of THP-1 cells?
1 answer-
Helpful?
-