product M1255, Calcein Blue to is used to stain cells, but it will stain living or dead cells. For a cell viability stain, one can use Calcein Blue AM. The difference between Calcein Blue and Calcein Blue AM is the methyl-iminodiaceitc acid group that chelates the divalent metal has acetoxymethyl esters on it in Calcein Blue AM. The esters are cleaved inside of viable cells, but remain on the molecule in the absence of active esterases. So, if you use M1255 it will stain all the cell whether alive or dead.
M1255
Calcein Blue
Powder
Sinonimo/i:
4-Methylumbelliferone-8-methyliminodiacetic acid
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1 G
CHF 105.00
10 G
CHF 505.00
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Cambia visualizzazione
1 G
CHF 105.00
10 G
CHF 505.00
About This Item
Formula empirica (notazione di Hill):
C15H15NO7
Numero CAS:
Peso molecolare:
321.28
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47
Prodotti consigliati
Nome del prodotto
Calcein Blue, fluorescent dye
Stato
powder
tecniche
titration: suitable
Colore
white to light yellow
Solubilità
1 M NaOH: 50 mg/mL
Fluorescenza
λex 322 nm; λem 445 nm in methanol
Compatibilità
suitable for indicator for EDTA titration
applicazioni
diagnostic assay manufacturing
hematology
histology
Temperatura di conservazione
room temp
Stringa SMILE
CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
InChI
1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
RJPSHDMGSVVHFA-UHFFFAOYSA-N
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Descrizione generale
Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
Applicazioni
Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Gloves
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Is Calcein Blue identical to Calcein Blue AM, and if not, what is the difference? Would it be compatible in a flourescent microscopy assay looking at %viability of THP-1 cells?
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