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C0875

Sigma-Aldrich

Calcein

Powder

Sinonimo/i:

Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon

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5 G
CHF 262.00
10 G
CHF 581.00
25 G
CHF 1’160.00

CHF 262.00


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Cambia visualizzazione
5 G
CHF 262.00
10 G
CHF 581.00
25 G
CHF 1’160.00

About This Item

Formula empirica (notazione di Hill):
C30H26N2O13
Numero CAS:
Peso molecolare:
622.53
Beilstein:
8181068
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47

CHF 262.00


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Nome del prodotto

Calcein, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

Descrizione

mixed isomers

Stato

powder

tecniche

titration: suitable

Colore

yellow to brown

Solubilità

NaOH: 50 mg/mL

Fluorescenza

λex 470 nm; λem 509 nm (pH 7.4)

Compatibilità

suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

InChI

DEGAKNSWVGKMLS-UHFFFAOYSA-N

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Applicazioni

Calcein has been used:
  • in whole cell staining[1]
  • in histomorphometry and micro-computed tomography[2]
  • to label active bone-forming surfaces[3]

Azioni biochim/fisiol

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions.[4] Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.[5]

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Questions

1–5 of 5 Questions  
  1. What are the excitation and emission wavelengths of Calcein, Product C0875?

    1 answer
    1. Conn's Biological Stains, 10th Edition states excitation is at 494 nm in aqueous solutions at pH 9; the emission maximum is 517 nm. Depending upon the pH of the solution, slightly different values have been reported in the literature.

      Helpful?

  2. Is there a reference for using Calcein, Product C0875, to study bone growth?

    1 answer
    1. Clement-Lacroix, P., et al. Osteoblasts are a new target for prolactin: analysis of bone formation in prolactin receptor knockout mice. Endocrinology, 140, 96-105 (1999).

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What is the solubility of Calcein, Product C0875?

    1 answer
    1. Calcein is soluble at a concentration of 50 mg/mL in 1M sodium hydroxide, yielding clear orange to brown solution.

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  5. What is the extinction coefficient of Calcein, Product C0875?

    1 answer
    1. For samples in 1 M sodium hydroxide: E1% = 1040 at 500-504 nm; E1% = 466 at 242-245 nm.

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