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L3289

Sigma-Aldrich

LYSIS SOLUTION FOR BLOOD

sufficient for 100 reactions, for molecular biology

Sinonimo/i:

Blood direct PCR, Blood lysis buffer, Extract-N-Amp blood direct PCR lysis solution

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.25
Prezzi e disponibilità al momento non sono disponibili

Grado

Molecular Biology
for molecular biology

Stato

liquid

impiego

sufficient for 100 reactions

Temperatura di conservazione

−20°C

Descrizione generale

Lysis solution for blood in combination with neutralization solution for blood (N9784) aids in rapid extraction and neutralization of DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This lysis solution is a component of the Extract-N-Amp and the REDExtract-N-Amp Blood PCR Kits

Applicazioni

Lysis solution for blood has been used for lysis of blood cells for DNA extraction prior to polymerase chain reaction (PCR) amplification.[1] It has also been used for the isolation of genomic DNA for CRISPR-Cas9 gene editing.[2][3]

Note legali

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Pittogrammi

Corrosion

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and Cellular Biology, MCB-00790 (2015)
Leandro S Thiago et al.
Haematologica, 99(1), 155-162 (2013-07-23)
The B-cell compartment in which multiple myeloma stem cells reside remains unclear. We investigated the potential presence of mature, surface-membrane immunoglobulin-positive B lymphocytes clonally related to the tumor bone marrow plasma cells among different subsets of peripheral blood B cells
Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and cellular biology, MCB-00790 (2015)
Aaron Zhong et al.
STAR protocols, 1(2) (2020-10-20)
Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made
Amber K Bowers et al.
Genetics, 164(4), 1345-1353 (2003-08-22)
To take advantage of available expressed sequence tags and genomic sequence, we have developed 64 PCR-based molecular markers in Chlamydomonas reinhardtii that map to the 17 linkage groups. These markers will allow the rapid association of a candidate gene sequence

Questions

  1. Does L3289 - Lysis Solution for blood come in a size larger than 2.5 mL. I have purchased a 10 mL size in the past but am not seeing that size as an option.

    1 answer
    1. Unfortunately, a 10 mL package size is not available at this time. The 25 mL package size has been discontinued. Should a custom volume quote be of interest, pricing and lead time may be requested at the link below:
      https://www.sigmaaldrich.com/US/en/services/custom-products/bulk-and-custom-services

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