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H5416

Sigma-Aldrich

FLT3 Ligand human

recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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About This Item

Numero MDL:
MFCD01634859
Codice UNSPSC:
12352202
NACRES:
NA.77

Origine biologica

human

Livello qualitativo

100

Ricombinante

expressed in HEK 293 cells

Saggio

≥95% (SDS-PAGE)

Forma fisica

lyophilized powder

Potenza

≤0.8 ng/mL EC50

Qualità

endotoxin tested

PM

monomer 24-30 kDa (glycosylated)

Confezionamento

pkg of 10 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

tecniche

cell culture | mammalian: suitable

Impurezze

≤1 EU/mg

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Human FMS-like tyrosine kinase 3 (FLT3) ligand is a homodimer consisting of two short-chain α-helical bundles. The molecular weight of FLT3 ligand is 44 kDa.

Azioni biochim/fisiol

FLT3 ligand binds to the cell-bound tyrosine kinase receptor and activates them.
HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. HumanKine FLT3 Ligand in human HEK 293 cells contributes to glycosylation.FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.

Stato fisico

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Risultati analitici

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Note legali

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots
Savvides S N, et al.
Nature Structural and Molecular Biology, 7(6), 486-486 (2000)
Nobuyuki Onai et al.
Annals of the New York Academy of Sciences, 1106, 253-261 (2007-03-16)
Flt3-ligand is a nonredundant cytokine in type I interferon-producing cell (IPC) and dendritic cell (DC) development. We demonstrated that IPC and DC differentiation potential is confined to Flt3(+)-hematopoietic progenitor cells, that Flt3-ligand drives development along both lymphoid and myeloid developmental
Structure-function analysis of FLT3 ligand-FLT3 receptor interactions using a rapid functional screen
Graddis T, et al.
The Journal of Biological Chemistry, 273(28), 17626-17633 (1998)
Chunaram Choudhary et al.
International journal of hematology, 82(2), 93-99 (2005-09-09)
Activating mutations of Fms-like tyrosine kinase 3 (Flt3) are the most common genetic lesions in acute myeloid leukemia (AML) and are present in approximately one third of AML patients. The 2 classes of Flt3 mutations are internal tandem duplications in

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