GE28-4082-53

HiTrap^® MabSelect^™

Cytiva 28-4082-53, pack of 5 × 1 mL

• Codice UNSPSC: 23151816
• NACRES: NA.56

Proprietà

• ligand: recombinant protein A (E. coli)
• Confezionamento: pack of 5 × 1 mL
• Produttore/marchio commerciale: Cytiva 28-4082-53
• Condizioni di stoccaggio: (20% Ehtanol); (20% Ethanol)
• Parametri: 42 psi
• Dimensioni del letto: 7 mm × 25 mm
• Volume del letto: 1 mL
• Diam. int. colonna: 7 mm
• Matrice: highly cross-linked agarose
• Dimens. media delle particelle: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• working range: 3-10
• Capacità: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• Compatibilità: suitable for bioprocess medium
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Applicazioni

Lab Purification using the MabSelect^™ Protein A tag for capture of antibodies.

Caratteristiche e vantaggi

• HiTrap^® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

Informazioni sulla sicurezza

• Pittogrammi: GHS02
• Avvertenze: Warning
• Indicazioni di pericolo: H226
• Consigli di prudenza: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Codice della classe di stoccaggio: 3 - Flammable liquids