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GE17-1279-03

rProtein A Sepharose™ Fast Flow

Cytiva 17-1279-03, pack of 200 mL

Sinonimo/i:

antibody affinity medium, antibody purification resin

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

Confezionamento

pack of 200 mL

Produttore/marchio commerciale

Cytiva 17-1279-03

Condizioni di stoccaggio

(20% Ehtanol)

Matrice

4% cross-linked agarose

Dimensione particelle

60-165 μm

Diametro medio

90 μm (d50v)

cleaning in place

2-11

Intervallo di esercizio

3-10

Capacità

~50 mg binding capacity (human lgG/ml medium)

Compatibilità

suitable for bioprocess medium

Temperatura di conservazione

2-8°C

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

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Certificati d'analisi (COA)

Lot/Batch Number

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Cell reports, 38(7), 110367-110367 (2022-02-17)
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Articoli

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocolli

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Vedi tutto

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