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G1279

Sigma-Aldrich

G 418

powder, suitable for plant cell culture, BioReagent

Sinonimo/i:

Antibiotico G418

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250 MG
CHF 106.00
1 G
CHF 333.00
5 G
CHF 1’170.00

CHF 106.00

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Cambia visualizzazione
250 MG
CHF 106.00
1 G
CHF 333.00
5 G
CHF 1’170.00

About This Item

Formula empirica (notazione di Hill):
C20H40N4O10 · 2H2SO4
Numero CAS:
108321-42-2
Peso molecolare:
692.71
Numero MDL:
MFCD00058314
Codice UNSPSC:
10171502
ID PubChem:
24895058
NACRES:
NA.72

CHF 106.00

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Nome del prodotto

G 418, suitable for plant cell culture, BioReagent

Nome Commerciale

BioReagent

Livello qualitativo

200

Stato

powder

Potenza

≥720 μg per mg (Dried basis)

tecniche

cell culture | plant: suitable

Colore

white to off-white

Solubilità

100 mg/mL (H2O clear)

Spettro attività antibiotica

Gram-negative bacteria

applicazioni

agriculture

Modalità d’azione

protein synthesis | interferes

Temperatura di conservazione

2-8°C

Stringa SMILE

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
UHEPSJJJMTWUCP-NKCAIAFTSA-N

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Categorie correlate

Descrizione generale

Chemical structure: aminoglycoside

Applicazioni

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Avvertenza

The product is stable for three years when stored at 2-8°C.

Nota sulla preparazione

G418 powder is soluble in water at 100 mg/mL.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H317,H334

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number

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Frida C Mohlin et al.
Molecular immunology, 65(2), 367-376 (2015-03-04)
Atypical hemolytic uremic syndrome (aHUS) is a disease of complement dysregulation, characterized by hemolytic anemia, thrombocytopenia and acute renal failure. Mutations in complement inhibitors are major risk factors for development of aHUS. The three aHUS patients reported in this study
Yan-Ping Li et al.
STAR protocols, 2(2), 100438-100438 (2021-04-27)
Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR)
Luis M Molinos-Albert et al.
Retrovirology, 11, 44-44 (2014-06-10)
The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals.We have quantified and
Elsa A Marquez et al.
PloS one, 10(7), e0131308-e0131308 (2015-07-07)
Ly49 receptors can be peptide selective in their recognition of MHC-I-peptide complexes, affording them a level of discrimination beyond detecting the presence or absence of specific MHC-I allele products. Despite this ability, little is understood regarding the properties that enable
Qifan Zheng et al.
Neuro-oncology, 16(9), 1229-1243 (2014-05-28)
As a commonly mutated form of the epidermal growth factor receptor, EGFRvIII strongly promotes glioblastoma (GBM) tumor invasion and progression, but the mechanisms underlying this promotion are not fully understood. Through gene manipulation, we established EGFRvIII-, wild-type EGFR-, and vector-expressing
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Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

Protocolli

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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