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FD500S

Destrano marcato con isotiocianato di fluoresceina

Name: Destrano marcato con isotiocianato di fluoresceina
Brand: SIGMA

average mol wt 500,000

Sinonimo/i:

FITC–destrano

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Informazioni su questo articolo

Numero CAS:

60842-46-8

UNSPSC Code:

12352201

NACRES:

NA.25

MDL number:

MFCD00131092

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Proprietà

biological source

synthetic (organic)

Quality Segment

200

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 500,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

color

yellow to orange

solubility

H₂O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

storage temp.

2-8°C

Descrizione

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 500 kDa, may be used as a cerebral spinal fluid (CSF) tracer. FITC-dextran-500 may be used to study endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability.

Destrano marcato con isotiocianato di fluoresceina per il possibile utilizzo negli studi di perfusione negli animali.

FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

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Questo articolo	FD2000S	FD40S	46945
Sigma-Aldrich FD500S Destrano marcato con isotiocianato di fluoresceina	Sigma-Aldrich FD2000S Destrano marcato con isotiocianato di fluoresceina	Sigma-Aldrich FD40S Destrano marcato con isotiocianato di fluoresceina	Sigma-Aldrich 46945 Destrano marcato con isotiocianato di fluoresceina
description average mol wt 500,000	description average mol wt 2,000,000	description average mol wt 40,000	description average mol wt 70,000, (FITC:Glucose = 1:250)
biological source synthetic (organic)	biological source synthetic (organic)	biological source synthetic (organic)	biological source synthetic
solubility H₂O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange	solubility H₂O: soluble 25 mg/mL	solubility H₂O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange	solubility H₂O: 50 mg/mL, hazy, orange to very deep orange
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
form powder	form powder	form powder	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
color yellow to orange	color yellow to orange	color yellow to dark orange	color light yellow to very dark yellow, light orange to very dark orange