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F5803

Sigma-Aldrich

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

Sinonimo/i:

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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50 MG
CHF 115.00

CHF 115.00

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50 MG
CHF 115.00

About This Item

Formula empirica (notazione di Hill):
C14H16N2O2 · 2HCl
Numero CAS:
20325-40-0
Peso molecolare:
317.21
Beilstein:
3917996
Numero CE:
243-737-5
Numero MDL:
MFCD00012488
Codice UNSPSC:
12171500
ID PubChem:
24894894
NACRES:
NA.47

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Stato

crystalline

Intervallo di pH

6.95-7.05

Punto di fusione

268 °C

Solubilità

water: 2.5 mg/mL, colorless

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

2-8°C

Stringa SMILE

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H
UXTIAFYTYOEQHV-UHFFFAOYSA-N

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Descrizione generale

A preweighed vial containing 50 mg o-Dianisidine.

Specificità

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Applicazioni

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
  • As substrate for the determination of serum ceruloplasmin from its oxidase activity.[1]
  • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.[2]
  • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).[3]
  • Myeloperoxidase activity assay.[4]

Attenzione

Probably carcinogenic.

Enzima

N° Catalogo
Descrizione
Determinazione del prezzo

Standard

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Descrizione
Determinazione del prezzo

Avvertenze

Danger

Indicazioni di pericolo

H302,H314,H350

Classi di pericolo

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificati d'analisi (COA)

Lot/Batch Number
0000345795
0000345795
SLCM0676
SLCF9565
SLCB2275

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Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride.
K H Schosinsky et al.
Clinical chemistry, 20(12), 1556-1563 (1974-12-01)
Wijitar Dungchai et al.
Analytica chimica acta, 674(2), 227-233 (2010-08-04)
We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for
Risheng Ye et al.
The Journal of clinical investigation, 128(3), 1178-1189 (2018-02-20)
The compensatory proliferation of insulin-producing β cells is critical to maintaining glucose homeostasis at the early stage of type 2 diabetes. Failure of β cells to proliferate results in hyperglycemia and insulin dependence in patients. To understand the effect of
G B Penner et al.
Journal of dairy science, 92(6), 2767-2781 (2009-05-19)
The objective of the study was to investigate the fractional rate of volatile fatty acid (VFA) absorption and the expression of genes encoding for transporters and enzymes involved in the absorption and metabolism of VFA in ruminal tissue when cattle
L O Chow et al.
Journal of dairy science, 91(4), 1534-1543 (2008-03-20)
Objectives of the study were to evaluate the effect of planting date on in vitro neutral detergent fiber digestibility (IVFD) of whole-crop barley (Hordeum vulgare) and its effects on productivity of lactating dairy cows. Two cultivars of barley were planted
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Protocolli

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

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