E6758

Ethylenediaminetetraacetic acid

anhydrous, crystalline, BioReagent, suitable for cell culture

• Sinonimo/i: (Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid
• Formula condensata: (HO₂CCH₂)₂NCH₂CH₂N(CH₂CO₂H)₂
• Numero CAS: 60-00-4
• Peso molecolare: 292.24
• Beilstein: 1716295
• Numero CE: 200-449-4
• Numero MDL: MFCD00003541
• Codice UNSPSC: 12161700
• eCl@ss: 39030907
• ID PubChem: 24894634
• NACRES: NA.25

Proprietà

• Grado: anhydrous
• Livello qualitativo: 200
• Nome Commerciale: BioReagent
• Saggio: ≥98.5%
• Forma fisica: crystalline
• Impiego in reazioni chimiche: reagent type: chelator
• tecniche: cell culture | mammalian: suitable
• pH: 2.5 (23 °C, 10 g/L)
• Punto di fusione: 250 °C (dec.) (lit.)
• Solubilità: 3 M NaOH: 100 mg/mL
• Densità: 1.46 g/cm³ at 20 °C
• Cationi in tracce: C: 40.7-41.5%; N: 9.3-9.8%
• Stringa SMILE: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20); KCXVZYZYPLLWCC-UHFFFAOYSA-N

Descrizione

Applicazioni

Ethylenediaminetetraacetic acid has been used:

• as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation
• as a medium component in the hepatic stellate cell (HSCs) culture
• as a component of homogenization buffer for protein extraction from eye tissues
• for stopping benzonase reaction in lysis buffer

Azioni biochim/fisiol

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood samples. EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia. EDTA along with tetracycline imparts serves as root conditioning agent and provides relief in gum infection. EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis. Calcium Disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H319
• Consigli di prudenza: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classi di pericolo: Eye Irrit. 2
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves