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D3035

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

Deoxyribonucleic acid from human placenta

Sinonimo/i:

DNA

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Informazioni su questo articolo

Numero CAS:
9007-49-2
UNSPSC Code:
41106310
NACRES:
NA.52
MDL number:
MFCD00130921
eCl@ss:
32160414
Biological source:
human placenta
Form:
buffered aqueous solution
Storage temp.:
−20°C
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biological source

human placenta

Quality Segment

200

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Application

Deoxyribonucleic acid from human placenta has been used:
  • to compare the efficiency of DNA quantification methods
  • as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
  • as an internal control to estimate the degree of fragmentation of circulating DNA
  • for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
  • to compare the 70-bp P5 exon sequence between DNA of different human and primate species
  • to calculate copy number of genes, relative to normal human tissue
  • in PCR reactions
For use in Southern hybridizations.

Other Notes

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

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Deoxyribonucleic acid from human placenta buffered aqueous solution, sexed, female

Sigma-Aldrich

D3035

Deoxyribonucleic acid from human placenta

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes Purified DNA from salmon testes. Absorption (0.001% solution in phosphate buffer, pH 7.0, 260 nm): ≥0.155.

Millipore

262012

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes

Plasmid DNA from E. coli RRI pUC18, buffered aqueous solution

Sigma-Aldrich

D4154

Plasmid DNA from E. coli RRI

Plasmid DNA from E. coli RRI pUC19, buffered aqueous solution

Sigma-Aldrich

D3404

Plasmid DNA from E. coli RRI

description

buffered aqueous solution, sexed, female

description

Purified DNA from salmon testes. Absorption (0.001% solution in phosphate buffer, pH 7.0, 260 nm): ≥0.155.

description

pUC18, buffered aqueous solution

description

pUC19, buffered aqueous solution

biological source

human placenta

biological source

-

biological source

Escherichia coli (RRI)

biological source

-

grade

Molecular Biology

grade

-

grade

Molecular Biology

grade

Molecular Biology

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

form

buffered aqueous solution

form

solid

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

shipped in

dry ice

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Questions

  1. Hi, Could you please clarify what is the concentration (and the volume) of the D3035 product. Thank you

    1 answer

    1. The concentration will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate:
      https://www.sigmaaldrich.com/product/sigma/d3035#product-documentation

      The volume will depend on the pack size ordered and the concentration.
      For instance, a 0.5 mg pack at 0.05 mg/ml will have a volume of at least 10ml.

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