GM1899A

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human unknown/unspecified
• Confezionamento: tube of 5 μg 98120701-DNA-5UG; pkg of vial of cells 98120701-1VL
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human lymphoblast, HPRT - deficient

Descrizione della linea cellulare

The lymphoblastoid cell line GM1899A has been derived from a Lesch-Nyhan patient. It is deficient for hypoxanthine-guanine phosphoribosyl transferase (HPRT) with a point mutation in the respective gene. This cell line is used as a feeder for extended-term culture of human T-lymphocytes (see LB lines in the collection).

Applicazioni

Feeder for T-lymphocyte culture

Terreno di coltura

Dutch Modified RPMI 1640 + 2mM Glutamine + 20mM Hepes + 2mM Sodium Pyruvate (NaPy) + 20% Heated Inactivated Foetal Bovine Serum (HI-FBS)

Mantenimento delle subcolture

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Do not dilute below 1 x 100,000 cells/ml, wash 2-3 times after resuscitation before seeding; disaggregate every few days by pipetting to avoid formation of large aggregates

Altre note

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