LB205

98062329, human blood, Lymphoblastoid

• Codice UNSPSC: 41106514

Proprietà

• product name: LB205, 98062329
• Origine biologica: human blood
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human lymphocytes, Xeroderma pigmentosum variant, EBV-transformed

Descrizione della linea cellulare

Derived from mononuclear cells from the peripheral blood of a 62 year old male. The patient was diagnosed with Xeroderma pigmentosum variant, illustrating that the disease is heterogeneous. T cells from the original sample have been used for UV irradiation studies and hprt mutant frequency. The cell line has been established by transformation with EBV. Fibroblasts from the same individual are designated XP11 BR.

Applicazioni

DNA repair deficiency studies, hprt mutation analysis

Terreno di coltura

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Mantenimento delle subcolture

Maintain cultures between 3-9 x 100,000 cells/ml dilute every 1-2 days to 3 x 100,000 cells/ml 5%

Altre note

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