BF-11

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: Rat x Mouse Hybridoma
• Confezionamento: tube of 5 μg 95050413-DNA-5UG; pkg of vial of cells 95050413-1VL
• ImmunoDon: Rat AC1 spleen cells
• Mieloma: Sp2/0 Ag-14
• Reattività: Anti-Erythropoietin, human
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat ACI spleen x Mouse Sp2/0 Ag-14

Descrizione della linea cellulare

The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.

Immunogeno

Human erythropoietin

Applicazioni

Immunological studies, isolation of erythropoietin by immuno-affinity methods.

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Terreno di coltura

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

Altre note

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