SK-N-DZ

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human nerve (bone marrow metastasis)
• Confezionamento: tube of 5 μg 94092305-DNA-5UG; pkg of vial of cells 94092305-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.
• Morfologia: Neuronal
• Prodotti: p53, moderate MDR-1 expression
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: metastasis
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human neuroblastoma

Descrizione della linea cellulare

Spontaneously transformed cells from a female patient with neuroblastoma. The tumour was derived from a bone marrow metastasis and is tumourigenic in nude mice. Used in the study of MIBG uptake in the diagnosis and treatment of neuroblastoma. MIBG is a derivative of the antihypertensive agent guanethidine and is used as a radiopharmaceutical. SK-N-DZ is characterised by low saturable kinetics and predominant linear pattern of uptake of MIBG, with a poor ability to maintain high intracellular levels of the drug.

Applicazioni

Tumour studies. MRD-1 resistance studies. Extremely sensitive to cis-retinoic acid.

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18

Terreno di coltura

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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