RENT RO3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: rat endometrium
• Confezionamento: tube of 5 μg 92092514-DNA-5UG; pkg of vial of cells 92092514-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Spindle shaped
• Prodotti: Not specified
• Recettori: Glucocorticoid
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

Descrizione della linea cellulare

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, uvomorulin, proenkephalin, desmin and connexin 43 markers.

Terreno di coltura

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time 35 h.

Altre note

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