RENT tsS1.1

92092515, rat endometrium, Cuboidal

• Codice UNSPSC: 41106514

Proprietà

• product name: RENT tsS1.1, 92092515
• Origine biologica: rat endometrium
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Cuboidal
• Prodotti: Not specified
• Recettori: Glucocorticoid
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

Descrizione della linea cellulare

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43 markers. The transforming agent is a ts mutant of SV 40 T-antigen (tsA58).

Terreno di coltura

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

Altre note

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