HT29 gluc C1

92012401, human colon, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: HT29 gluc C1, 92012401
• Origine biologica: human colon
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian colon adenocarcinoma

Descrizione della linea cellulare

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

Terreno di coltura

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO₃) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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