M-MSV-BALB/3T3

90030802, mouse embryo, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: M-MSV-BALB/3T3, 90030802
• Origine biologica: mouse embryo
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 57
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse BALB/3T3 embryo, Moloney sarcoma virus transformed

Descrizione della linea cellulare

Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.

Applicazioni

Transformation and virus studies: M-MSV, MuLV

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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