MCA-RH 7777

90021504, rat liver, Hepatocyte

• Codice UNSPSC: 41106514

Proprietà

• product name: MCA-RH 7777, 90021504
• Origine biologica: rat liver
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Hepatocyte
• Prodotti: Alpha-foetoprotein
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat Buffalo hepatoma

Descrizione della linea cellulare

Derived from the Morris hepatoma 7777 (obtained from a male Buffalo strain rat). The original tumour was induced with N-2 fluorenylphthalamic acid. Particularly interesting for oncogeny studies since the cells produce α-fetoprotein (AFP).

Applicazioni

Tumourigenicity and oncogeny studies

Terreno di coltura

Swimm′s S-77 + 4mM Glutamine + 20% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split semi-confluent cultures 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. NB: Cells are shed from the monolayer before confluency is achieved.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.