BRL-1

94121649, rat liver, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: BRL-1, 94121649
• Origine biologica: rat liver
• Modalità di accrescimento: Adherent
• Cariotipo: Diploid or pseudodiploid
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat epithelial cells

Descrizione della linea cellulare

Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.

Applicazioni

Feeder cells for co-culture with freshly isolated hepatocytes.

Terreno di coltura

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Mantenimento delle subcolture

Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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