The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask
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