LTK-

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011432, mouse connective tissue, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse connective tissue
• Confezionamento: tube of 5 μg 85011432-DNA-5UG; pkg of vial of cells 85011432-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C34/An connective tissue

Descrizione della linea cellulare

Sub-line of BUdR resistant strain of the L-M mouse line (Sigma Catalogue number. 87032401), which in turn was derived from L929 (Sigma Catalogue number. 85011425). TK- cells cannot grow in HAT medium. Stable TK- mutants are only maintained if the cells are kept for several passages in growth medium containing BUdR.

Applicazioni

Somatic cell hybridisation and gene transfer studies

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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