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C7527

Choline chloride

Name: Choline chloride
Brand: SIGMA
Price: 64.1 CHF

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

Sinonimo/i:

(2-Hydroxyethyl)trimethylammonium chloride

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Voi/SKU	Disponibilità	Prezzo
100 g	Per conoscere la disponibilità, visualizza il carrello	CHF 64.10
500 g	Per conoscere la disponibilità, visualizza il carrello	CHF 198.00
1 kg	Per conoscere la disponibilità, visualizza il carrello	CHF 352.00

Informazioni su questo articolo

Formula condensata:

(CH₃)₃N(Cl)CH₂CH₂OH

Numero CAS:

67-48-1

Peso molecolare:

139.62

UNSPSC Code:

12352205

NACRES:

NA.75

PubChem Substance ID:

24892968

EC Number:

200-655-4

Beilstein/REAXYS Number:

3563126

MDL number:

MFCD00011721

CHF 64.10

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Proprietà

biological source

synthetic (organic)

Quality Segment

300

product line

BioReagent

assay

≥98%

form

crystals

mol wt

M_w 139.62 g/mol

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

color

white

pH

5.0-6.5 (25 °C, 140 g/L)

mp

302-305 °C (dec.) (lit.)

solubility

H₂O: 50 mg/mL

SMILES string

[Cl-].C[N+](C)(C)CCO

InChI

1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key

SGMZJAMFUVOLNK-UHFFFAOYSA-M

Descrizione

General description

Listen to an Audio Overview
Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD). It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions.

Choline acetyltransferase substrate

Application

Choline chloride has been used:

as a component of choline acetyltransferase (ChAT) buffer and for choline uptake in cultured human neuroblastoma (SK-N-SH) cells
for choline standard curve generation for quantifying phospholipase D (PLD) activity
as a component of slice solution for the dissection of entorhinal-hippocampal brain slices

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Questo articolo	PHG0021	W450001	C1879
Sigma-Aldrich C7527 Choline chloride	SAFC PHG0021 Choline chloride	Sigma-Aldrich W450001 Choline chloride	Sigma-Aldrich C1879 Choline chloride
description BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%	description -	description FCC	description ≥98%
technique(s) cell culture \| plant: suitable, cell culture \| insect: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) cell culture \| mammalian: suitable
assay ≥98%	assay -	assay -	assay ≥98%
biological source synthetic (organic)	biological source synthetic	biological source synthetic	biological source synthetic
form crystals	form powder	form powder, crystals or chunks	form powder or crystals
solubility H₂O: 50 mg/mL	solubility water: soluble 140 g/L	solubility -	solubility -
color white	color white	color white	color colorless to white

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Classe di stoccaggio

11 - Combustible Solids

Che succede?

WGK 1

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

DPI (dispositivi di protezione individuale)

dust mask type N95 (US), Eyeshields, Gloves

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Certificati d'analisi (COA)

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Questions

1–3 of 3 Questions

2 years ago

Choline chloride will be melted in water for addition to the medium. Can you please tell me the proper storage method, such as -30°C freezing ay -4°C refrigeration?

1 answer
1. Technical Support
  
  2 years ago
  
  This product is soluble in water at 50 mg/mL. The storage and stability conditions for this product upon reconstitution have not been determined.
  
  Helpful?
2 years ago

Hi, is it possible to thaw choline chloride in PBS instead of water and add it to the medium?

1 answer
1. Technical Support
  
  2 years ago
  
  This product is soluble in water, tested at 50 mg/mL. Solubility in PBS has not been determined in-house. Literature suggests that choline chloride can be prepared with PBS. For reference, see https://en-cdn.bio-protocol.org/pdf/Bio-protocol807.pdf?rel_link=YmlvLXByb3RvY29sLm9yZy9lbi9icGRldGFpbD9pZD04MDcmdHlwZT0w
  
  Helpful?
3 years ago

Hi, could you please provide the product information sheet for Choline chloride.

1 answer
1. Technical Support
  
  3 years ago
  
  There is no specific product information sheet offered for Choline Chloride. Please see the PROPERTIES section of the product page for details. Links to a sample Certificate of Analysis and a Certificate of Origin are provided below:
  Certificate of Analysis
  https://www.sigmaaldrich.com/certificates/COFA/C7/C7527/C7527-100G________SLBB8921V_.pdf
  Certificate of Origin
  https://www.sigmaaldrich.com/US/en/coo/undefined/C7527/SLBB8921V
  
  Helpful?

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