C3131

Citocromo c

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

• Sinonimo/i: CYC, CytC
• Numero CAS: 9007-43-6
• UNSPSC Code: 12352202
• EC Number: 232-700-9
• NACRES: NA.61
• MDL number: MFCD00130890
• Form: powder
• Assay: ≥95% based on Mol. Wt. 12,327 basis
• Biological source: bovine heart
• Mol wt: 12327 Da

Proprietà

• biological source: bovine heart
• Quality Segment: 200
• assay: ≥95% based on Mol. Wt. 12,327 basis
• form: powder
• mol wt: 12327 Da
• storage condition: (Tightly closed Dry)
• technique(s): cell culture | mammalian: suitable
• impurities: 0.40-0.50% Iron (anhydrous)
• solubility: water: 10 mg/mL, dark red-brown
• UniProt accession no.: P62894
• storage temp.: −20°C
• Gene Information: cow ... CYC1(512500)

Descrizione

General description

Research area: Apoptosis

Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria. It is encoded in the nucleus as apo-cytochrome C. Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids. It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres.

Application

Cytochrome C from bovine heart has been used:

• as a component of the assay solution for testing the activity of respiratory chain complexes III and IV in the mitochondria of human osteosarcoma and human hepatocarcinoma cell lines
• to determine the cytochrome c oxidase activity in mitochondria of human aortic endothelial cells
• as a component of the cyt C oxidase reaction solution during in situ enzyme staining of cyt C oxidase in mice kidney tissues

Biochem/physiol Actions

Cytochrome C (Cyt C) is involved in the activation of caspase during the caspase-dependent apoptosis intrinsic pathway that triggers programmed cell death through apoptosis. It shows lipid-binding activity through its interaction with cardiolipin that accounts for the peroxidase activity of Cyt C. Cyt C binds to heme via the help of cytochrome c heme lyase that enables its release into the mitochondrial intermembrane space.

La rapida interconversione del citocromo c tra stati ferrosi e ferrici lo rende un efficiente trasportatore biologico di elettroni. Svolge un ruolo fondamentale nelle ossidazioni cellulari, sia nelle piante che negli animali. Considerato un anello universale della catena respiratoria, costituisce il ponte di elettroni essenziale tra i substrati respirabili e l'ossigeno.

Preparation Note

Prepared with acetic acid without using TCA.

Other Notes

Per maggiori informazioni sul citocromo c e il trasporto di elettroni visitare il sito www.sigma-aldrich.com/enzymeexplorer.

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• Che succede?: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable
• DPI (dispositivi di protezione individuale): Eyeshields, Gloves, type N95 (US)