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B6149

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl phosphate disodium salt

chromogenic, ≥98% (HPLC), powder

Sinonimo/i:

5-BCIP disodium salt, BCIP®, X-phosphate disodium salt

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About This Item

Formula condensata:
C8H4BrClNO4P · 2Na
Numero CAS:
102185-33-1
Peso molecolare:
370.43
Numero MDL:
MFCD00036757
Codice UNSPSC:
12352204
ID PubChem:
24891897
NACRES:
NA.83

product name

5-Bromo-4-chloro-3-indolyl phosphate disodium salt, ≥98% (HPLC)

Saggio

≥98% (HPLC)

Forma fisica

powder

Solubilità

H2O: 20 mg/mL
DMF: insoluble

Temperatura di conservazione

−20°C

Stringa SMILE

[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12

InChI

1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
OAZUOCJOEUNDEK-UHFFFAOYSA-L

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Categorie correlate

Applicazioni

5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Substrati

Histochemical substrate for alkaline phosphatase.

Note legali

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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NBT-BCIP® solution BioReagent, suitable as substrate for alkaline phosphatase in dot blots

Sigma-Aldrich

72091

NBT-BCIP® solution

BCIP®/NBT solution, premixed

Supelco

B6404

BCIP®/NBT solution, premixed

SIGMAFAST™ BCIP®/NBT tablet

Sigma-Aldrich

B5655

SIGMAFAST™ BCIP®/NBT

NBT 4-Nitro blue tetrazolium chloride, solution

Roche

11383213001

NBT

5-bromo-4-cloro-3-indolil fosfato ≥95.0% (HPLC)

Sigma-Aldrich

16670

5-bromo-4-cloro-3-indolil fosfato

Manli Qi et al.
Journal of bacteriology, 193(10), 2498-2509 (2011-03-29)
The Chlamydia-specific hypothetical protein CT795 was dominantly recognized by human antisera produced during C. trachomatis infection but not by animal antisera raised against dead chlamydia organisms. The immundominant region recognized by the human antibodies was mapped to the N-terminal fragment
P E Høyer et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(8), 855-863 (1996-08-01)
We describe a quantitative histochemical method for demonstration of five N-acetyl-glucosamine binding lectins in the syncytiotrophoblast of human term placenta. The method employs biotinylated lectins and alkaline phosphatase-conjugated avidin. The alkaline phosphatase activity is detected by using 5-bromo-4-chloro-indoxyl phosphate as
Shailesh Kumar et al.
PLoS genetics, 15(6), e1008219-e1008219 (2019-06-27)
Fes1 is a conserved armadillo repeat-containing Hsp70 nucleotide exchange factor important for growth at high temperature, proteasomal protein degradation and prion propagation. Depleting or mutating Fes1 induces a stress response and causes defects in these processes that are ascribed solely
Thermal preference ranges correlate with stable signals of universal stress markers in Lake Baikal endemic and Holarctic amphipods
Axenov-Gribanov D, et al
PLoS ONE, 11(10), e0164226-e0164226 (2016)
The endosomal protein endotubin is required for enterocyte differentiation
Cox CM, et al.
Cellular and molecular gastroenterology and hepatology, 5(2), 145-156 (2018)
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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