B3275
Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse
clone RG-16, purified immunoglobulin, buffered aqueous solution
Sinonimo/i:
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
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0.2 ML
CHF 160.00
CHF 160.00
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Cambia visualizzazione
0.2 ML
CHF 160.00
About This Item
Codice UNSPSC:
12352203
NACRES:
NA.46
CHF 160.00
Check Cart for Availability
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
biotin conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
secondary antibodies
Clone
RG-16, monoclonal
Stato
buffered aqueous solution
Reattività contro le specie
rabbit
Non deve reagire con
human
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
tecniche
direct ELISA: 1:60,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500
western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
western blot: 1:200,000
Isotipo
IgG1
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Categorie correlate
Descrizione generale
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
Specificità
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Applicazioni
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Azioni biochim/fisiol
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens.
Stato fisico
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
M Penkowa et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 20(8), 1174-1189 (2000-08-19)
To study the importance of metallothionein-I and -II (MT-I+II) for brain inflammation and regeneration, the authors examined normal and MT-I+II knock-out (MT-KO) mice subjected to a cortical freeze injury. Normal mice showed profound neurodegeneration, inflammation, and gliosis around the injury
Albert Quintana et al.
Journal of neuroscience research, 82(5), 701-716 (2005-11-04)
Tumor necrosis factor-alpha (TNF-alpha) is one of the mediators dramatically increased after traumatic brain injury that leads to the activation, proliferation, and hypertrophy of mononuclear, phagocytic cells and gliosis. Eventually, TNF-alpha can induce both apoptosis and necrosis via intracellular signaling.
Takeshi Hayashi et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 25(1), 41-53 (2005-01-29)
The endoplasmic reticulum (ER), which plays a role in apoptosis, is susceptible to oxidative stress. Because superoxide is produced in the brain after ischemia/reperfusion, oxidative injury to this organelle may be implicated in ischemic neuronal cell death. Activating transcription factor-4
Takeshi Hayashi et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 23(10), 1117-1128 (2003-10-04)
The endoplasmic reticulum (ER), which plays important roles in apoptosis, is susceptible to oxidative stress. Because reactive oxygen species (ROS) are robustly produced in the ischemic brain, ER damage by ROS may be implicated in ischemic neuronal cell death. We
T Moos et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(6), 591-603 (1996-06-01)
Despite the presence of a blood-brain barrier (BBB), plasma proteins have been detected intraneuronally in regions with axonal projections confined to the CNS. This finding raises the question of whether plasma proteins are taken up from the brain interstitium or
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