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B2904

Sigma-Aldrich

Bluo-Gal

reagent for selection of recombinant bacterial clones

Sinonimo/i:

5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal

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About This Item

Formula empirica (notazione di Hill):
C14H16BrNO6
Numero CAS:
97753-82-7
Peso molecolare:
374.18
Beilstein:
1550140
Numero MDL:
MFCD00063691
Codice UNSPSC:
12352200
ID PubChem:
24891417
NACRES:
NA.85

Grado

for molecular biology

Sterilità

non-sterile

Saggio

≥98% (HPLC)

Forma fisica

powder

tecniche

nucleic acid detection: suitable

Solubilità

DMF: soluble

Compatibilità

suitable for substrate for β-galactosidase

Temperatura di conservazione

−20°C

Stringa SMILE

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
LINMATFDVHBYOS-MBJXGIAVSA-N

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Descrizione generale

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Applicazioni

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Principio

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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