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B2661
Anti-fd Bacteriophage−Biotin antibody produced in rabbit
~3.5 mg/mL, IgG fraction of antiserum
Sinonimo/i:
Anti-fd Bacteriophage
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0.5 ML
CHF 628.00
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0.5 ML
CHF 628.00
About This Item
Codice UNSPSC:
12352203
NACRES:
NA.46
Prodotti consigliati
Origine biologica
rabbit
Coniugato
biotin conjugate
Forma dell’anticorpo
IgG fraction of antiserum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Concentrazione
~3.5 mg/mL
tecniche
indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Specificità
The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
Immunogeno
fd bacteriophage
Applicazioni
Anti-fd Bacteriophage-Biotin antibody produced in rabbit was used to access immunoglobulin content using bead-based immunoassays.
Stato fisico
Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
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Human myoblasts and muscle-derived SP cells.
Grace K Pavlath et al.
Methods in molecular medicine, 107, 97-110 (2004-10-20)
Robert Burger et al.
Lab on a chip, 12(7), 1289-1295 (2012-02-16)
We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic
Matthew Robert Tomkins et al.
Sensors (Basel, Switzerland), 15(1), 1047-1059 (2015-01-13)
A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the "fingerprinting" capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres)
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What is the Department of Transportation shipping information for this product?
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What is the pH of Product B2661, Anti-fd Bacteriophage, in solution?
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There is no pH test specification for product B2261 on a lot-to-lot basis. A one-time measurement of the pH of a 2% solution was 1.9. It has been observed that this material will precipitate from phosphate buffer solutions.
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The description states Product B2661, Anti-fd Bacteriophage, may be used for staining DNA, chromosomes and nuclei, and for fluorescence microscopy or flow cytometry. Are there other uses for the product?
1 answer-
The product has also been used for the detection of apoptosis, applied as a label to live cells, as a component of BrdU cell cycle investigation.
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What is the expected solution stability for aqueous solutions of Product B2661, Anti-fd Bacteriophage?
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Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.
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Will chromosomes that are dividing or replicating stain with Product B2661, Anti-fd Bacteriophage?
1 answer-
No; chromosomes that are dividing or replicating will not stain with this dye.
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Which is more cell permeable, H33342 or H33258?
1 answer-
H33342 has been found to be about 10 times more cell-permeable than H33258. See Science, 282(5387), 296-298, (1998).
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What is the specificity for H33342?
1 answer-
The bisBenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. Reference: Loontiens, F. G., et al., Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)] and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities. Biochemistry, 29, p. 9029 (1990).
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When using Product B2661, Anti-fd Bacteriophage, which is the preferred Hoechst stain for chromosomes?
1 answer-
Hoechst No. 33258 and not Hoechst No. 33342 is the preferable dye for chromosomal staining.
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