A9941

2,2′-azino-bis(acido 3-etilbenzotiazolina-6-solfonico)

chromogenic, tablet

• Sinonimo/i: AzBTS-(NH₄)₂, Diammonio 2,2′-azino-bis(3-etilbenzotiazolina-6-solfonato)
• Formula empirica (notazione di Hill): C₁₈H₂₄N₆O₆S₄
• Numero CAS: 30931-67-0
• Peso molecolare: 548.68
• NACRES: NA.21
• PubChem Substance ID: 24891410
• UNSPSC Code: 12352204
• EC Number: 250-396-6
• MDL number: MFCD00010404
• Beilstein/REAXYS Number: 7329461

Proprietà

• Nome del prodotto: 2,2′-azino-bis(acido 3-etilbenzotiazolina-6-solfonico), tablet, 10 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• technique(s): protein detection: suitable
• solubility: deionized water: 100 mL
• storage temp.: room temp
• SMILES string: [NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
• InChI: 1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
• InChI key: OHDRQQURAXLVGJ-AXMZSLBLSA-N

Descrizione

General description

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:

• For a reaction with laccase or bilirubin oxidase
• For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide

A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).
ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound

Application

Il 2,2′-azino-bis(acido 3-etilbenzotiazolina-6-solfonico) è un substrato della perossidasi adatto all′uso nelle procedure ELISA. Il substrato dà luogo a un prodotto finale solubile di colore verde che può essere identificato con lettura spettrofotometrica a 405 nm. La reazione può essere arrestata con sodio dodecil solfato (SDS) all′1%. Consigliato per procedure ELISA (in micropozzetti); non indicato per applicazioni su membrana.

• 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).†
• ABTS is also used to measure glucose concentrations in different solutions including blood serum.
• The food industry uses ABTS to measure the antioxidant capacities within different foods.
• Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
• It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Preparation Note

Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable
• DPI (dispositivi di protezione individuale): dust mask type N95 (US), Eyeshields, Gloves
• Che succede?: WGK 2
• Hazard Classifications: Eye Irrit. 2
• Pittogrammi: GHS07
• Parola di avvertenza: Warning
• Codici di pericolo: H319
• Avvertenze di sicurezza: P264 - P280 - P305 + P351 + P338 - P337 + P313