A9205

Albumina sierica bovina

30% in saline, fatty acid free, aseptically filled

• Sinonimo/i: Albumina, Soluzione di BSA
• Numero CAS: 9048-46-8
• Numero MDL: MFCD00130384
• Codice UNSPSC: 12352202
• NACRES: NA.27

Proprietà

• Origine biologica: bovine
• Livello qualitativo: 200
• Sterilità: aseptically filled
• Saggio: 28-32% protein basis (biuret)
• Stato: solution
• Purificato mediante: cold ethanol fractionation
• non contiene: stabilizer or preservative
• Origine: USA origin
• Concentrazione: 28-32% protein (biuret); 30% in saline
• free fatty acid content: ≤0.01%
• tecniche: ELISA: suitable; electrophoresis: suitable; western blot: suitable
• Impurezze: ≤0.01% free fatty acid
• N° accesso UniProt: P02769
• Attività estranea: BT virus, none detected; VSV virus, none detected
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: bovine ... ALB(280717)

Descrizione

Descrizione generale

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.[1]

Applicazioni

Bovine Serum Albumin solution has been used:

• as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells[2]
• as a component of hepatocyte recovery medium[3]
• as a component of fusion and activation buffers for in vitro reconstructed sheep embryos[4]

Azioni biochim/fisiol

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis.[5] It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation.[6] Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry.[1] BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.[7]

Nota sulla preparazione

Initial fractionation by cold alcohol precipitation

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)