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A7510

Adenosine 5′-Triphosphatase from porcine cerebral cortex

lyophilized powder, ≥0.3 units/mg protein, pH 7.8

Sinonimo/i:

ATP phosphohydrolase, ATPase

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Voi/SKUDisponibilitàPrezzo
5 units
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CHF 183.00
5 x 5 units
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CHF 683.00

Informazioni su questo articolo

Numero CAS:
9000-83-3
UNSPSC Code:
12352204
NACRES:
NA.54
Numero CE:
3.6.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00135671
Specific activity:
≥0.3 units/mg protein, pH 7.8
Biological source:
Porcine cerebral cortex

CHF 183.00

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biological source

Porcine cerebral cortex

Quality Segment

description

Sodium and Potassium-Activated

form

lyophilized powder

specific activity

≥0.3 units/mg protein, pH 7.8

composition

Protein, 5.0-25.0% biuret

technique(s)

inhibition assay: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

Application

ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity .

Biochem/physiol Actions

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells .

Physical form

Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl

Preparation Note

Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).

Other Notes

One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na+, K+, and Mg2+.

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Questo articolo
A7646A9187I0879
Adenosine 5′-Triphosphatase from porcine cerebral cortex lyophilized powder, ≥0.3 units/mg protein, pH 7.8

Sigma-Aldrich

A7510

Adenosine 5′-Triphosphatase from porcine cerebral cortex

Apirasi ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Sigma-Aldrich

A7646

Apirasi

Adenosina 5′-trifosfato sale di magnesio ≥95%, bacterial

Sigma-Aldrich

A9187

Adenosina 5′-trifosfato sale di magnesio

Inosine 5′-triphosphate trisodium salt ≥95%

Sigma-Aldrich

I0879

Inosine 5′-triphosphate trisodium salt

description

lyophilized powder, ≥0.3 units/mg protein, pH 7.8

description

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

description

≥95%, bacterial

description

≥95%

biological source

Porcine cerebral cortex

biological source

potato

biological source

bacterial

biological source

-

specific activity

≥0.3 units/mg protein, pH 7.8

specific activity

-

specific activity

-

specific activity

-

Gene Information

pig ... ATP1A1(397481), ATP1B1(396898)

Gene Information

-

Gene Information

-

Gene Information

human ... HRAS(3265)

technique(s)

inhibition assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

lyophilized powder

form

lyophilized powder (partially purified)

form

powder

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Classe di stoccaggio

11 - Combustible Solids

Che succede?

WGK 3

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

DPI (dispositivi di protezione individuale)

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number

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