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A4862

Sigma-Aldrich

α-Amylase from Bacillus sp.

liquid

Sinonimo/i:

1,4-α-D-glucan glucanohydrolase, 1,4-alpha-D-glucan glucanohydrolase, Termamyl® 300L, alpha-amylase, endoglucanase

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50 ML
CHF 121.00
250 ML
CHF 292.00

CHF 121.00


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Cambia visualizzazione
50 ML
CHF 121.00
250 ML
CHF 292.00

About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

CHF 121.00


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Origine biologica

Bacillus sp.

Stato

liquid

Temperatura di conservazione

2-8°C

Descrizione generale

α-Amylase belongs to glycosyl hydrolase family 13.[1] It comprises of A, B and C domains. Domain A has two aspartic acid and a glutamic acid as catalytic residues. Domain B possesses Ca2+ and Na+ binding sites.[2]

Applicazioni

α-Amylase from Bacillus sp. has been used in the digestion of lyophilized microalgae sample.[3] It has also been used to evaluate α-amylase inhibitory activity in marine algae samples[4] and Myrmecodia platytyrea.[5]

Azioni biochim/fisiol

α-Amylase hydrolyzes the polysaccharides starch, malto-oligosaccharides at the α-d-(1,4)-glucosidic linkages to α-anomeric products.[1] It has wide applications in industries including food, pharmaceutical, textile and paper.[6]

Definizione di unità

One unit will liberate 1.0 mg of maltose from starch in 3 minutes at pH 6.9 at 20 deg C.

Note legali

A product of Novozyme Corp.
Termamyl is a registered trademark of Novozymes Corp.

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Improving the reversibility of thermal denaturation and catalytic efficiency of Bacillus licheniformis alpha-amylase through stabilizing a long loop in domain B
Li Z, et al.
PLoS ONE, 12(3), e0173187-e0173187 (2017)
Microalgae Characterization for Consolidated and New Application in Human Food, Animal Feed and Nutraceuticals
Molino A, et al.
International Journal of Environmental Research and Public Health, 15(11), 2436-2436 (2018)
Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose
Kagawa M, et al.
Journal of Bacteriology, 185(23), 6981-6984 (2003)
High-performance thin-layer chromatographic methods in the evaluation of the antioxidant and anti-hyperglycemic activity of Myrmecodia platytyrea as a promising opportunity in diabetes treatment
Agatonovic-Kustrin S, et al.
Journal of Chromatography A, 1530, 192-196 (2017)
Snezana Agatonovic-Kustrin et al.
Marine drugs, 17(3) (2019-03-06)
Marine organisms produce an array of biologically active natural products, many of which have unique structures that have not been found in terrestrial organisms. Hence, marine algae provide a unique source of bioactive compounds. The present study investigated 19 marine

Protocolli

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Questions

  1. Can I dilute the alpha amylase (A4862) in distilled/deionized water or should I use any specific buffer for dilution?

    1 answer
    1. This product may be diluted further using ultrapure water. Please see the link below to review the enzymatic assay method for this product:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-a-amylase

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