A4605
Anti-iASPP antibody, Mouse monoclonal
clone LXO49.3, purified from hybridoma cell culture
Sinonimo/i:
Anti-Inhibitory Member of the Apoptosis-stimulating Protein of p53 Family, Anti-RAI, Anti-Rel-associated Inhibitor
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100 μL
CHF 631.00
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100 μL
CHF 631.00
About This Item
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
LXO49.3, monoclonal
Stato
buffered aqueous solution
PM
antigen ~100 kDa
Reattività contro le specie
mouse, human
Concentrazione
~2 mg/mL
tecniche
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of human osteogenic sarcoma (U-2-OS)
Isotipo
IgG1
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... PPP1R13L(10848)
mouse ... Ppp1r13l(333654)
Descrizione generale
Monoclonal Anti-iASPP (mouse IgG1 isotype) is derived from the hybridoma LXO49, produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding residues of human iASPP. The ASPP family of proteins contains three members, ASSP1, ASSP2 and iASPP. iASPP is also known as REL associated inhibitor (RAI). iASPP exists as a 100 kDa form and as a 70 kDa form (iASPP/RAI). It is found both in the nucleus and cytoplasm, while iASPP/RAI is mainly cytosolic. PPP1R13L (protein phosphatase 1 regulatory subunit 13 like) codes for iASPP, that is located on human chromosome 19q13.
iASPP is an inhibitory member of the apoptosis-stimulating protein of p53 (ASPP) family. It is an oncoprotein that associates with Ras, E1A and E7 to transform cells.
Applicazioni
Monoclonal Anti-iASPP antibody produced in mouse has been used in:
- immunoblotting
- immunocytochemistry
- immunoprecipitation
Mouse monoclonal Anti-iASPP antibody can be used for western blot (0.1-0.2μg/mL using total cell extract of human osteogenic sarcoma), microarray, immunoprecipitation and immunocytochemistry.
Azioni biochim/fisiol
PPP1R13L (protein phosphatase 1 regulatory subunit 13 like)/iASPP inhibits p53-mediated cell death. It interacts and inhibits NFkB p65 RelA. It may act as a therapeutic target for bladder cancer (BC). It modulates cell proliferation and apoptosis. iASPP plays a key role in breast cancer and acute leukemia.
Stato fisico
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Ariel M Wilson et al.
PloS one, 9(4), e94175-e94175 (2014-04-10)
The transcription factor p53 mediates the apoptosis of post-mitotic neurons exposed to a wide range of stress stimuli. The apoptotic activity of p53 is tightly regulated by the apoptosis-stimulating proteins of p53 (ASPP) family members: ASPP1, ASPP2 and iASPP. We
Wenjie Ge et al.
Cell death discovery, 7(1), 195-195 (2021-07-28)
p53 and NF-κBp65 are essential transcription factors (TFs) in the cellular response to stress. Two signaling systems can often be entwined together and generally produce opposing biological outcomes in a cell context-dependent manner. Inhibitor of apoptosis-stimulating protein of p53 (iASPP)
The miR-124-p63 feedback loop modulates colorectal cancer growth
Liu K, et al.
Oncotarget, 8(17), 29101-29101 (2017)
A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity
Kramer D, et al.
Cell Death & Disease, 6(2), e1634-e1634 (2015)
Wenjie Ge et al.
Cancer cell, 32(5), 561-573 (2017-10-17)
Reactive oxygen species (ROS) have emerged as important signaling molecules that play crucial roles in carcinogenesis and cytotoxic responses. Nrf2 is the master regulator of ROS balance. Thus, uncovering mechanisms of Nrf2 regulation is important for the development of alternative
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