A4362
Activin A active human
Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
Sinonimo/i:
Erythroid differentiation protein, Inhibin beta A chain; Activin beta-A chain
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Prodotti consigliati
Origine biologica
human
Livello qualitativo
Ricombinante
expressed in Nicotiana
Saggio
>97% (SDS-PAGE)
Forma fisica
lyophilized
Potenza
≤5 ng/mL ED50
PM
27.4 kDa (disulfide-linked homodimers of two βA chains, each containing 116 amino residues)
Confezionamento
pkg of 5 μg
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles
Impurezze
Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... INHBA(3624)
Categorie correlate
Amino Acid Sequence
HisHisHisHisHisHisGlyLeuGluCysAspGlyLysValAsnIleCysCysLysLysGlnPhePheValSerPheLysAspIleGlyTrpAsnAspTrpIleIleAlaProSerGlyTyrHisAlaAsnTyrCysGluGlyGluCysProSerHisIleAlaGlyThrSerGlySerSerLeuSerPheHisSerThrValIleAsnHisTyrArgMetArgGlyHisSerProPheAlaAsnLeuLysSerCysCysValProThrLysLeuArgProMetSerMetLeuTyrTyrAspAspGlyGlnAsnIleIleLysLysAspIleGlnAsnMetIleValGluGluCysGlyCysSer
Descrizione generale
Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin A has two 116 amino acids residues βA subunits (βA-βA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.
As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.
As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.
Stato fisico
Lyophilized from a Tris HCl 0.05 M buffer at pH 7.4
Ricostituzione
Lyophilized protein should be reconstituted in water to a concentration of 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.
Risultati analitici
The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation.
ED50: ≤ 5 ng/mL
ED50: ≤ 5 ng/mL
Altre note
Extinction Coefficient: E0.1% = 1.27 (A 280 nm)
p.I: 7.27
p.I: 7.27
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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