A3429 and A3179 are the same solution but in different package sizes. No dilution is required for these products, but some customers may prefer a slightly weaker acid differentiation solution. The differentiation time may vary due to staining time and the thickness of the tissue sections being stained.
A3429
Differentiation Solution
for the differentiation of regressive hematoxylin stains
Sinonimo/i:
Acid Alcohol
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
4 L | Per conoscere la disponibilità, visualizza il carrello | CHF 238.00 |
Informazioni su questo articolo
Servizio Tecnico
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Permettici di aiutartiQuality Segment
form
solution
shelf life
40 mo.
color
colorless
application(s)
hematology
histology
storage temp.
room temp
General description
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
Application
Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:
Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
Biochem/physiol Actions
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.
Features and Benefits
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
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Questo articolo | |||
|---|---|---|---|
| description for the differentiation of regressive hematoxylin stains | description - | description NIST® SRM® 2897a, nominal mass fraction 2% | description anhydrous, ≥99% |
| color colorless | color colorless | color - | color - |
| application(s) hematology | application(s) hematology | application(s) environmental | application(s) - |
| form solution | form solution | form - | form liquid |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| storage temp. room temp | storage temp. room temp | storage temp. - | storage temp. - |
| shelf life 40 mo. | shelf life 40 mo. | shelf life - | shelf life - |
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
target_organs
Eyes,Central nervous system
Classe di stoccaggio
3 - Flammable liquids
flash_point_f
71.6 °F
flash_point_c
22 °C
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Do Differentiation Solutions A3179 and A3429 need to be diluted before use?
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