71196

Sodium acetate solution

BioUltra, Molecular Biology, ~3 M in H₂O

• Formula condensata: CH₃CO₂Na
• Numero CAS: 127-09-3
• Peso molecolare: 82.03
• NACRES: NA.21
• PubChem Substance ID: 57652229
• eCl@ss: 39021906
• UNSPSC Code: 12161700
• MDL number: MFCD00012459
• Beilstein/REAXYS Number: 3595639

Proprietà

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioUltra
• form: liquid
• concentration: ~3 M in H₂O
• technique(s): precipitation assay: suitable
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• pH: 7.0-8.0 (25 °C, 3 M in H₂O)
• anion traces: chloride (Cl^-): ≤10 mg/kg, phosphate (PO₄^3-): ≤5 mg/kg, sulfate (SO₄^2-): ≤20 mg/kg
• cation traces: Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤50 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
• λ: 3 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.030, λ: 280 nm A_max: ≤0.024
• SMILES string: [Na+].CC([O-])=O
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• InChI key: VMHLLURERBWHNL-UHFFFAOYSA-M

Descrizione

Application

Sodium acetate solution has been used:

• in DNA extraction
• in reverse crosslinking and DNA purification
• to precipitate Gata6 (GATA binding protein 6) cDNA as a part of high-purity plasmid preparation

Biochem/physiol Actions

Sodium acetate serves as a fixed base. It is useful to rectify acidosis among acidotic patients. Orginally, sodium acetate is used as dialysate for hemodialysis, but is known to cause cardiovascular instability.

Other Notes

Basic method of DNA precipitation with ethanol.

Informazioni sulla sicurezza

• Classe di stoccaggio: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves