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Documenti fondamentali

51456

Sigma-Aldrich

Urea

BioUltra, for molecular biology, 99% (T)

Sinonimo/i:

Carbamide, Carbonildiamide

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500 G
CHF 113.00
2.5 KG
CHF 391.00

CHF 113.00


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500 G
CHF 113.00
2.5 KG
CHF 391.00

About This Item

Formula condensata:
NH2CONH2
Numero CAS:
Peso molecolare:
60.06
Beilstein:
635724
Numero CE:
Numero MDL:
Codice UNSPSC:
12352111
ID PubChem:
NACRES:
NA.25

CHF 113.00


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Grado

Molecular Biology
for molecular biology

Nome Commerciale

BioUltra

Saggio

99% (T)

Stato

powder or crystals

Impurezze

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.1% biuret
<0.005% formaldehyde (HCHO)

Residuo alla calcinazione

≤0.01% (as SO4)

Perdita

≤0.1% loss on drying, 20 °C (HV)

pH

8.0-10.0 (20 °C, 8 M in H2O)

Punto di fusione

132-135 °C (lit.)

Solubilità

H2O: 8 M at 20 °C, clear, colorless

Densità

1.335 g/mL at 25 °C (lit.)

Anioni in tracce

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤10 mg/kg

Cationi in tracce

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

Stringa SMILE

NC(N)=O

λ

8 M in H2O

Assorbanza UV

λ: 260 nm Amax: ≤0.03
λ: 280 nm Amax: ≤0.02

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
XSQUKJJJFZCRTK-UHFFFAOYSA-N

Informazioni sul gene

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Applicazioni

Usata per la denaturazione di proteine e come blando agente di solubilizzazione per proteine insolubili o denaturate. Utilizzata per rinaturare proteine da campioni già denaturati con cloruro di guanidinio 6 M come corpi di inclusione. Può essere impiegata con idrocloruro di guanidina e ditiotreitolo (DTT) nella ripiegatura di proteine denaturate nella loro forma nativa o attiva.

Altre note

Denaturing reagent[1]; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides[2][3][4]

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Certificati d'analisi (COA)

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Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98% formamide or 7 M urea.
T Maniatis et al.
Methods in enzymology, 65(1), 299-305 (1980-01-01)
R. Poulson et al.
The Ribonucleic Acids, 333-333 (1977)
A Paulus et al.
Electrophoresis, 14(1-2), 27-35 (1993-01-01)
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Protocolli

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

Questions

1–2 of 2 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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