This product may be stored at room temperature.
47671
Formamide
BioUltra, for molecular biology, ≥99.5% (T)
Sinonimo/i:
Amide C1, Ammide formica
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250 ML
CHF 84.20
1 L
CHF 227.00
2.5 L
CHF 501.00
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Cambia visualizzazione
250 ML
CHF 84.20
1 L
CHF 227.00
2.5 L
CHF 501.00
About This Item
Formula condensata:
HCONH2
Numero CAS:
Peso molecolare:
45.04
Beilstein:
505995
Numero CE:
Numero MDL:
Codice UNSPSC:
12352111
ID PubChem:
NACRES:
NA.25
Grado:
for molecular biology
Saggio:
≥99.5% (T)
tecniche:
FISH: suitable
hybridization: suitable
protein extraction: suitable
hybridization: suitable
protein extraction: suitable
applicazioni:
genomic analysis
P. ebollizione:
210 °C (lit.)
Tensione di vapore:
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
30 mmHg ( 129 °C)
Prodotti consigliati
Grado
for molecular biology
Livello qualitativo
Densità del vapore
1.55 (vs air)
Tensione di vapore
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
Nome Commerciale
BioUltra
Saggio
≥99.5% (T)
Stato
liquid
Temp. autoaccensione
932 °F
tecniche
FISH: suitable
hybridization: suitable
protein extraction: suitable
Impurezze
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
Residuo alla calcinazione
≤0.1%
Indice di rifrazione
n20/D 1.447 (lit.)
n20/D 1.447
pH
7-9 (25 °C, 1 M in H2O)
P. ebollizione
210 °C (lit.)
Punto di fusione
2-3 °C (lit.)
Solubilità
H2O: 10 M at 20 °C, clear, colorless
Densità
1.134 g/mL at 25 °C (lit.)
Anioni in tracce
chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg
Cationi in tracce
Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg
Stringa SMILE
NC=O
λ
10 M in H2O
Assorbanza UV
λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1
applicazioni
genomic analysis
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
ZHNUHDYFZUAESO-UHFFFAOYSA-N
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Descrizione generale
Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.
Applicazioni
Formamide has been used:
- as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
- in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
- in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
- to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
- as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent
La formamide destabilizza le doppie eliche dell′acido nucleico e può essere usata, di solito, a una concentrazione del 50%, in protocolli di ibridazione che richiedono temperature di ibridazione inferiori.
Azioni biochim/fisiol
Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.
Caratteristiche e vantaggi
- Versatile and adaptable for a wide variety of laboratory and research applications
- BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations
Altre note
For additional information on our range of Biochemicals, please complete this form.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Carc. 2 - Repr. 1B - STOT RE 2 Oral
Organi bersaglio
Blood
Codice della classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
305.6 °F
Punto d’infiammabilità (°C)
152 °C
Dispositivi di protezione individuale
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
Min Chen et al.
Prenatal diagnosis, 35(6), 534-543 (2015-02-03)
To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel
Jing Wang et al.
The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)
A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest
Vishnu Priya Bollampalli et al.
PLoS pathogens, 11(10), e1005206-e1005206 (2015-10-07)
The transport of antigen from the periphery to the draining lymph node (DLN) is critical for T-cell priming but remains poorly studied during infection with Mycobacterium bovis Bacille Calmette-Guérin (BCG). To address this we employed a mouse model to track
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What temperature should this product be stored at?
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What temperature should this product be stored at?
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This product may be stored at room temperature.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What expiry does Formamide get?
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The product specification sheet for this product contains a recommended retest date period of roughly 11 months after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
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Are aqueous solutions of Formamide, Product 47671, stable?
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In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).
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What is the best way to deionize Formamide, Product 47671?
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Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.
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What is the molarity of the neat liquid Formamide, Product 47671?
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Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to the chemicals encyclopedia published by the Royal Society of Chemistry, 12th edition.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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