3023
AptaFluor® SAH Methyltransferase Assay
Sinonimo/i:
Methyltransferase Activity Assay
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 1 ea | Per conoscere la disponibilità, visualizza il carrello | CHF 460.00 | |
| 1 ea | Per conoscere la disponibilità, visualizza il carrello | CHF 1’280.00 |
Informazioni su questo articolo
NACRES:
NA.84
UNSPSC Code:
41122100
Servizio Tecnico
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Permettici di aiutartishipped in
dry ice
storage temp.
−70°C
General description
The AptaFluor® SAH Methyltransferase TR-FRET Assay is a universal assay for enzymes that convert S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH), with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The assay can be used for all enzymes within the histone methyltransferase (HMT) and DNA methyltransferase (DNMT) families. The assay is designed specifically for high-throughput screening (HTS), with a simple mix-and-read format and 2 liquid addition steps. It is run in an endpoint mode: methyltransferase (MT) enzyme reactions are quenched with a universal stop reagent (provided) and then the detection reagents are added.
The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
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The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
- Simple, mix-and-read format ideal for HTS.
- Robust MT detection under initial velocity conditions (=20% conversion of SAM to SAH).
- Accommodates SAM concentrations as low as 100 nM and as high as 5 μM.
- Far-red tracer further minimizes interference from fluorescent compounds and light scattering.
View full Transcreener® product list
Physical form
Kit with buffered aqueous solutions
Preparation Note
3023-B = 100 assay, 96-well
3023-1K = 1,000 assay, 384-well
3023-1K = 1,000 assay, 384-well
Legal Information
AptaFluor is a registered trademark of BellBrook Labs
Transcreener is a registered trademark of BellBrook Labs
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Questo articolo | |||
|---|---|---|---|
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| 229164-5G | 04061832339054 |
| 229164-25G | 04061832339047 |
| 3023-1K | 04061835357116 |
| 3023-B | 04061835357444 |