14562

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester

BioReagent, suitable for fluorescence

• Sinonimo/i: BCECF-AM
• Formula empirica (notazione di Hill): C₃₉H₃₆O₁₉
• Peso molecolare: 808.69
• UNSPSC Code: 12352108
• NACRES: NA.32
• MDL number: MFCD01862636

Proprietà

• product line: BioReagent
• Quality Segment: 100
• form: solid
• solubility: DMF: soluble, DMSO: soluble, acetonitrile: soluble
• fluorescence: λ_ex 482 nm; λ_em 528 nm in 0.1 M Tris pH 8.0 (esterase)
• suitability: suitable for fluorescence
• storage temp.: 2-8°C

Descrizione

Application

An uncharged, nonfluorescent molecule that is useful for noninvasive bulk loading of cell suspension because it is capable of permeating the cell membrane. Cellular nonspecific esterases cleave its lipophilic blocking groups, resulting in a charged, fluorescent form of the molecule. Compared to the parent compound, this charged derivative leaks out of the cell at a much slower rate. Utilized for pH measurements in perfused tissues, intercellular spaces, mammalian cells, plant cells, bacteria, and yeast. Also, employed to investigate in assays for cellular functional properties such as adhesion, multi-drug resistance, viability and cytotoxicity, apoptosis and chemotaxis.

Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• Che succede?: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable
• DPI (dispositivi di protezione individuale): Eyeshields, Gloves, type N95 (US)