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NGLYF-RO

Roche

N-Glycosidase F

Name: N-Glycosidase F
Brand: ROCHE
Price: 555 CHF

PNGase F of Flavobacterium meningosepticum, recombinant from E. coli

Sinonimo/i:

glycosidase

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A voi/SKU	Disponibilità	Prezzo
0.1 mL	Per conoscere la disponibilità, visualizza il carrello	CHF 555.00
0.25 mL	Per conoscere la disponibilità, visualizza il carrello	CHF 1100.00

Informazioni su questo articolo

UNSPSC Code:

12352204

Numero CE:

3.5.1.52 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

~25000 units/mg protein

Assay:

≥90% (SDS-PAGE)

Recombinant:

expressed in E. coli

CHF 555.00

Per conoscere la disponibilità, visualizza il carrello

Servizio Tecnico

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Proprietà

recombinant

expressed in E. coli

conjugate

(N-linked)

assay

≥90% (SDS-PAGE)

form

solution

specific activity

~25000 units/mg protein

packaging

pkg of 0.1 mL (11365169001 [100 U]), pkg of 0.25 mL (11365177001 [250 U])

manufacturer/tradename

Roche

optimum pH

7.0-8.0

storage temp.

−20°C

Descrizione

General description

Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine amidase

Application

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit.[1][2][3] The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.

Physical form

Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.

Legal Information

The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.

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Questo articolo	NGLYFL-RO	P9120	P7367
Roche NGLYF-RO N-Glycosidase F	Roche NGLYFL-RO N-Glicosidasi F	Sigma-Aldrich P9120 PNGase F from Elizabethkingia meningoseptica	Sigma-Aldrich P7367 PNGase F from Elizabethkingia meningoseptica
description PNGase F of Flavobacterium meningosepticum, recombinant from E. coli	description recombinant form of the gene from Flavobacterium meningosepticum	description recombinant, expressed in E. coli, set of 100 units nanomolar unit	description BioReagent, ≥95% (SDS-PAGE)
assay ≥90% (SDS-PAGE)	assay ≥90% (SDS-PAGE)	assay -	assay ≥95% (SDS-PAGE)
specific activity ~25000 units/mg protein	specific activity >25000 units/mg protein	specific activity ≥10 units/mg protein	specific activity -
form solution	form lyophilized	form -	form powder
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant -
optimum pH 7.0-8.0	optimum pH 7.0-8.0	optimum pH -	optimum pH ~8.6
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Classe di stoccaggio

12 - Non Combustible Liquids

wgk

WGK 1

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No data available

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