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10884308001

Roche

CPRG

Chlorophenol red-β-D-galactopyranoside

Sinonimo/i:

Chlorophenol Red-β-D-galactopyranoside, chlorophenol red-β-d-galactopyranoside, CPRG, Chlorophenol Red-β-D-galactoside

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About This Item

Formula empirica (notazione di Hill):
C25H22Cl2O10S
Numero CAS:
Peso molecolare:
585.41
Numero MDL:
Codice UNSPSC:
12352204
ID PubChem:
Prezzi e disponibilità al momento non sono disponibili

Livello qualitativo

Saggio

96% (HPLC)

Stato

powder

Confezionamento

pkg of 250 mg

Produttore/marchio commerciale

Roche

Condizioni di stoccaggio

protect from light

Intervallo di pH

6-8

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C (−15°C to −25°C)

Stringa SMILE

OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
YOUWVNCQJOMMEU-AETRGLENSA-N

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Descrizione generale

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength dye which is commonly used for colorimetric assays.[1] The reaction product has a dark red color and is soluble in water. It can be evaluated photometrically at 574 or 578 nm.
The chemical formula on the label represents the monosodium salt.

Applicazioni

CPRG has been used as a substrate for β-galactosidase assay.[2][3][4]
Colorimetric determination of β-Gal activity in cell extracts and ELISA.[3][5]

Azioni biochim/fisiol

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.[6]

Qualità

Contaminant: <0.1% chlorophenol red

Sequenza

CPRG is an approximately 1:1 mixture of the isomers.

Nota sulla preparazione

Working concentration: 5 mmol/l
Storage conditions (working solution): Reconstituted solution is stable for one day at 2 to 8 °C

Stoccaggio e stabilità

Store powder dry at -15 to -25 °C; stock solutions at 2–8 °C protected from light.

Altre note

For life science research only. Not for use in diagnostic procedures.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available


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Els Vanderperren Wim Demaré Ronny Blust Kris Cooreman Peter Bossier
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 6(5), 375-380 (2001-01-01)
The finding that a variety of chemicals display oestrogenic activity has resulted in the development of in vitro and in vivo assays to assess oestrogenic activity. One such assay, the yeast oestrogen assay (YES) makes use of recombinant yeast cells
Mallika Ghosh et al.
Bio-protocol, 2(24), e305-e305 (2012-12-20)
Antigen cross presentation is important for effective immune responses to tumors and viral infections. Dendritic cells are professional antigen presenting cells and are unique in their ability to cross-present exogenous antigens on MHC class I molecules and activate antigen specific
Matthias A Fügi et al.
Journal of lipid research, 55(5), 929-938 (2014-03-15)
Sterols are an essential class of lipids in eukaryotes, where they serve as structural components of membranes and play important roles as signaling molecules. Sterols are also of high pharmacological significance: cholesterol-lowering drugs are blockbusters in human health, and inhibitors
Clémence Sicard et al.
Analytical and bioanalytical chemistry, 406(22), 5395-5403 (2014-06-29)
We report on a new fluorimetric assay for β-galactosidase (β-gal) and faecal coliform bacteria that utilizes a long-wavelength dye, chlorophenol red-β-D-galactopyranoside (CPRG), that has been widely used for colorimetric assays. The novel feature of this new assay is the unexpected
Andrés Alloatti et al.
Bio-protocol, 6(22) (2017-02-28)
Antigen presentation by MHC class I molecules, also referred to as cross-presentation, elicits cytotoxic immune responses. In particular, dendritic cells (DC) are the most proficient cross-presenting cells, since they have developed unique means to control phagocytic and degradative pathways. This

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