10745782001
Roche
DNA, lambda
from bacteriophage lambda cl857 Sam 7
Sinonimo/i:
lambda dna
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About This Item
Codice UNSPSC:
41106310
Prodotti consigliati
Livello qualitativo
Forma fisica
solution
PM
32,300 kDa
Confezionamento
pkg of 1 mL
Produttore/marchio commerciale
Roche
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles
Concentrazione
250 μg/mL
Impurezze
RNA, none detected
nuclease, none detected
Colore
colorless
Solubilità
water: miscible
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C (−15°C to −25°C)
Categorie correlate
Descrizione generale
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Applicazioni
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Qualità
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Sequenza
Chain Length 48,502 bp
Stato fisico
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Stoccaggio e stabilità
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
No data available
Punto d’infiammabilità (°C)
No data available
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