10109045001
Roche
Pyruvate Kinase (PK)
from rabbit muscle
Sinonimo/i:
PK
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About This Item
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Prodotti consigliati
Origine biologica
rabbit muscle
Livello qualitativo
Stato
solution
Attività specifica
~200 units/mg protein (At 25 °C with PEP as the substrate.)
Confezionamento
pkg of 1 mL (10 mg)
Produttore/marchio commerciale
Roche
pH ottimale
7.0-7.5
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C (−15°C to −25°C)
Categorie correlate
Descrizione generale
Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate.[1]
Specificità
Specific activity: Approximately 200 U/mg at +25°C with PEP as the substrate.
Applicazioni
Pyruvate Kinase (PK) has been used in the kinase assay.[4]
Azioni biochim/fisiol
Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. The enzyme also exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.[5]
Qualità
Contaminants: <0.001% GK, <0.002% HK, “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each.
Stato fisico
Solution in 50% glycerol (v/v), pH approximately 6
Nota sulla preparazione
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
No data available
Punto d’infiammabilità (°C)
No data available
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