10105031001
Roche
β-Galactosidase
from E. coli overproducer
Sinonimo/i:
β-galactosidase
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About This Item
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Origine biologica
Escherichia coli
Livello qualitativo
Stato
suspension
PM
540 kDa
Confezionamento
pkg of 1,500 U
Produttore/marchio commerciale
Roche
Concentrazione
5.00 mg/mL
tecniche
activity assay: suitable
pH ottimale
7
Compatibilità
suitable for UV spectrophotometry and general use
applicazioni
life science and biopharma
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
β-Galactosidase or β-D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K+ or Na+ and Mg2+ to be fully active. β-Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with β-d-galactopyranosides.[1]
At 37 °C with 2-nitrophenyl-β-D-galactoside as the substrate, approximately 30 U/mg at 25 °C with lactose as the substrate; standardized with BSA.
Specificità
Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide.
Applicazioni
β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA).[2] Use β-Galactosidase to produce a calibration curve in enzymatic assays.
Qualità
Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each
Stato fisico
Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline
Nota sulla preparazione
The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose.
In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galactopyranoside.
In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galactopyranoside.
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
No data available
Punto d’infiammabilità (°C)
No data available
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