PC35
Anti-p53 (Ab-7) (Pantropic) Sheep pAb
liquid, Calbiochem®
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About This Item
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
sheep
Livello qualitativo
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
liquid
contiene
≤0.1% sodium azide as preservative (Anti-p53 and Anti-Sheep IgG only)
Reattività contro le specie
human, rat, mouse
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
Isotipo
IgG
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... TP53(7157)
Descrizione generale
Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 and SVT2 cells and breast carcinoma tissue.
Sheep polyclonal antibody supplied as diluted serum. Recognizes the ~53 kDa wild-type or mutant p53 protein. Supplied with normal sheep serum and biotinylated rabbit anti-sheep IgG.
This Anti-p53 (Ab-7) (Pantropic) Sheep pAb is validated for use in Frozen Sections, Immunoblotting, ICC, Paraffin Sections, IP for the detection of p53 (Ab-7) (Pantropic).
Immunogeno
Human
recombinant, human p53 protein
Applicazioni
Frozen Sections (1:500)
Immunoblotting (1:2500)
Immunocytochemistry (1:500)
Paraffin Sections (1:500, pepsin or heat pre-treatment required)
Immunoprecipitation (chromatin, see application references)
Componenti
Anti-p53 antisera, normal sheep serum, and biotinylated rabbit anti-sheep IgG
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
200 µl diluted (1:4) anti-p53 sheep serum, 500 µl undiluted normal sheep serum, and 100 µl biotinylated rabbit anti-sheep IgG.
Risultati analitici
Positive Control
A431 or SVT2 cells or breast carcinoma tissue
A431 or SVT2 cells or breast carcinoma tissue
Altre note
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Recommended dilutions for the biotinylated rabbit anti-sheep secondary are 1:25,000-1:200,000 for immunoblotting and 1:10,000 for immunocytochemistry and paraffin sections. For immunoblotting, do not add normal serum, non-fat dried milk, culture medium or other potential sources of biotin to the working solutions (i.e. block with 2-5% fatty acid free BSA). Use streptavidin-horseradish peroxidase (Cat. No. OR03L) or streptavidin-alkaline phosphatase (Cat. No. OR04L) at 10 ng/ml to attach an enzyme to the biotinylated secondary antibody, then visualize using standard colorimetric or chemiluminescent reagents. To maximize sensitivity of immunoblots, preconcentrate samples by immunoprecipitation with Cat. No. OP03, OP09, or OP43, then immunoblot using Cat. No. PC35 and chemiluminescent detection. Antibodies should be titrated for optimal results in individual systems.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
10 - Combustible liquids
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M Ciciarello et al.
The Journal of biological chemistry, 276(22), 19205-19213 (2001-05-29)
Growing evidence indicates a central role for p53 in mediating cell cycle arrest in response to mitotic spindle defects so as to prevent rereplication in cells in which the mitotic division has failed. Here we report that a transient inhibition
A Li et al.
Biology of reproduction, 76(3), 362-367 (2006-11-10)
The expression of TRP53 in blastocysts that had been cultured from the zygote stage in vitro for 90 h was compared with that in blastocysts collected from the uterus in C57BL6 (B6) and in F1 hybrid (B6CBF1) strain mice. In
Joshua D Dowell et al.
Biochimica et biophysica acta, 1773(3), 358-366 (2007-01-19)
p193/CUL7 is an E3 ubiquitin ligase initially identified as an SV40 Large T Antigen binding protein. Expression of a dominant interfering variant of mouse p193/CUL7 (designated 1152stop) conferred resistance to MG132- and etoposide-induced apoptosis in U2OS cells. Immune precipitation/Western analyses
Timothy A Sutton et al.
American journal of physiology. Renal physiology, 295(6), F1666-F1677 (2008-09-26)
Ischemia-reperfusion injury (IRI) is a common cause of acute kidney injury (AKI) and is characterized by widespread tubular and microvascular damage. The tumor suppressor p53 is upregulated after IRI and contributes to renal injury in part by promoting apoptosis. Acute
Vashe Chandrakanthan et al.
Reproductive biology and endocrinology : RB&E, 4, 61-61 (2006-11-23)
In the mouse, embryo culture results in a characteristic phenotype of retarded embryo preimplantation development and reduced numbers of cells within embryos. The expression of TRP53 is central to the regulation of the cell's capacity to proliferate and survive. In
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