OP13
Anti-N-Myc Mouse mAb (NCM II 100)
liquid, clone NCM II 100, Calbiochem®
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.43
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
NCM II 100, monoclonal
Forma fisica
liquid
non contiene
preservative
Reattività contro le specie
human, mouse
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
avoid repeated freeze/thaw cycles
Isotipo
IgG1
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... MYCN(4613)
Descrizione generale
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.
Recognizes the N-myc protein and its cleavage product in IMR5 cells.
This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.
Immunogeno
Human
recombinant, human, N-myc fusion protein, expressed in E. coli
Applicazioni
Frozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample, see application references)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample, see application references)
Confezionamento
Please refer to vial label for lot-specific concentration.
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
In 50 mM sodium phosphate buffer, 50% glycerol.
Ricostituzione
Following initial thaw, aliquot and freeze (-20°C).
Risultati analitici
Positive Control
IMR5 cells
IMR5 cells
Altre note
Brondyk, W.H. 1991. Oncogene6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen.20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.
Slamon, D.J., et al. 1986. Science232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.
Brodeur, G.M., et al. 1984. Science224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.
Kohl, N.E., et al. 1983. Cell35, 603.
Schwab, M., et al. 1983. Nature305, 245.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen.20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.
Slamon, D.J., et al. 1986. Science232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.
Brodeur, G.M., et al. 1984. Science224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.
Kohl, N.E., et al. 1983. Cell35, 603.
Schwab, M., et al. 1983. Nature305, 245.
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
H P Wang et al.
Journal of virology, 72(3), 2192-2198 (1998-03-14)
Cell line WH44KA is a highly malignant woodchuck hepatoma cell line. WH44KA cells contain a single woodchuck hepatitis virus (WHV) DNA integration in the 3' untranslated region of exon 3 of the woodchuck N-myc1 gene. The highly rearranged WHV DNA
S K Radhakrishnan et al.
Oncogene, 27(5), 694-699 (2007-08-29)
We have previously described the identification of a nucleoside analog transcriptional inhibitor ARC (4-amino-6-hydrazino-7-beta-D-ribofuranosyl-7H-pyrrolo[2,3-d]-pyrimidine-5-carboxamide) that was able to induce apoptosis in cancer cell lines of different origin. Here, we report the characterization of ARC on a panel of neuroblastoma cell
James Chappell et al.
Genes & development, 27(7), 725-733 (2013-04-18)
Suppression of extracellular signal-regulated kinase (ERK) signaling is an absolute requirement for the maintenance of murine pluripotent stem cells (mPSCs) and requires the MYC-binding partner MAX. In this study, we define a mechanism for this by showing that MYC/MAX complexes
Luca Montemurro et al.
Cancer research, 79(24), 6166-6177 (2019-10-17)
Approximately half of high-risk neuroblastoma is characterized by MYCN amplification. N-Myc promotes tumor progression by inducing cell growth and inhibiting differentiation. MYCN has also been shown to play an active role in mitochondrial metabolism, but this relationship is not well
Giovanni Perini et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(34), 12117-12122 (2005-08-12)
N-Myc is a transcription factor that forms heterodimers with the protein Max and binds gene promoters by recognizing a DNA sequence, CACGTG, called E-box. The identification of N-myc target genes is an important step for understanding N-Myc biological functions in
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