MABT530

Anti-N-cadherin Antibody, clone 6A9.2

clone 6A9.2, from mouse

• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 6A9.2, monoclonal
• Reattività contro le specie: rat, human
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001783
• N° accesso UniProt: P19022
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CDH2(1000)

Descrizione

Descrizione generale

Cadherin-2 (UniProt P19022; also known as CD325, CDw325, N-cadherin, Neural cadherin) is encoded by the CDH2 (also known as CDHN, NCAD) gene (Gene ID 1000) in human. N-Cadherin is a single-pass type I membrane protein that plays a role in calcium-dependent cell adhesion. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. Cadherins are synthesized as precursor proteins that are proteolytically cleaved to generate functional, mature proteins. Newly synthesized proN-cadherin (a.a. 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. Cadherins are composed of an extracellular domain (a.a. 160-724 of human N-cadherin) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (a.a. 725-745 of human N-cadherin), and a conserved cytoplasmic domain (a.a. 746-906 of human N-cadherin) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis.

Specificità

Clone 6A9.2 targets an epitope within N-cadherin extracellular cadherin 1 domain.

Immunogeno

GST/His-tagged recombinant human N-cadherin extracellular cadherin 1 domain fragment.

Applicazioni

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected N-cadherin in human cervical cancer, adrenal gland, and liver tissue sections.

Research Category
Cell Structure

This mouse monoclonal Anti-N-cadherin antibody, clone 6A9.2, Cat. No. MABT530 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of N-cadherin.

Qualità

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected N-cadherin in 10 µg of C6 rat glioma cell lysate.

Descrizione del bersaglio

~140 kDa observed. Target band size appears larger than the calculated molecular weights of 99.81/97.23/82.03 kDa (prepro-/pro-/mature human N-cadherin) and 99.69/97.24/81.91 kDa (prepro-/pro-/mature rat N-cadherin) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: 04-1126

Stato fisico

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1