MABN466

Anti-Ankyrin-G Antibody, clone N106/36

clone N106/36, from mouse

• Sinonimo/i: Ankyrin-3, ANK-3, Ankyrin-G
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: N106/36, monoclonal
• Reattività contro le specie: rat
• tecniche: immunohistochemistry: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_066267
• N° accesso UniProt: Q12955
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: rat ... Ank3(361833)

Descrizione

Descrizione generale

Ankyrins are a family of spectrin-binding proteins that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel, and the Na/K ATPase, as well as neuronal cell adhesion molecules related to L1. In the nervous system, three different ankyrins are known to exist; ankyrinB, ankyrinG, and ankyrinR. All three ankyrins contain an N-terminal membrane binding domain, an internal domain capable of binding spectrin, and a C-terminal regulatory domain which is the target of alternative splicing. AnkyrinB, the predominant neuronal ankyrin includes two isoforms of ~220 kDa and ~440 kDa. The ~220 kDa alternatively spliced isoform is the major form present in the adult brain, while the ~440 kDa form is preferentially expressed in the developing neonatal brain. Three forms of ankyrinG (~190 kDa, ~270 kDa, ~480 kDa) are localized in axonal initial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched. Ankyrins comprise ~0.5%-1% of the total membrane protein in the adult vertebrate brain.

Specificità

Other homologies: Human (92% sequence homology), and Mouse (87% sequence homology).

Immunogeno

Recombinant protein corresponding to human Ankyrin-G.

Applicazioni

Anti-Ankyrin-G Antibody, clone N106/36 is a highly specific mouse monoclonal antibody & that targets Ankyrin & has been tested in IHC.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Ankyrin-G in rat cerebral cortex tissue.

Immunohistochemistry Analysis: A representative lot detected Ankyrin-G in rat optic nerve tissue.

Immunofluorescence Analysis: A representative lot detected Ankyrin-G in adult rat cortex tissue.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Ankyrin-G in a rat model of TLE mEC layer II neurons (Hargus, N. J., et al. (2011). Neurobiol Dis. 41(2):361-376.)

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualità

Evaluated by Immunohistochemistry in rat hippocampus tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Ankyrin-G in rat hippocampus tissue.

Descrizione del bersaglio

480 kDa calculated

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2a κ in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Rat hippocampus tissue

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable